Journal: Oncotarget

Article Title: FAM83B-mediated activation of PI3K/AKT and MAPK signaling cooperates to promote epithelial cell transformation and resistance to targeted therapies

doi:

Figure Lengend Snippet: (a) Western analysis of HCC1937 and MDA468 cells expressing shRNAs targeting GFP (G) or FAM83B (B) for FAM83B and GAPDH. (b) HCC1937 cells expressing shRNAs targeting GFP (G) or FAM83B (B) were plated and cell number quantified 5 days later. (c and d) HCC1937 cells expressing shRNAs targeting GFP (G) or FAM83B (B) were assessed for AIG or were grown as 3-dimensional (3D) cultures in laminin-rich basement membrane (lrBM) for 10 days. Pictures were taken and acini number determined using MetaMorph image quantitation software. (e and f) HCC1937 and MDA468 cells expressing shRNAs targeting GFP (G) or FAM83B (B) were grown in lrBM for 10 days and western analysis was performed for p-AKT (S473), p-ERK1/2 (Thr 202/Tyr 204), ERK1/2, AKT, and GAPDH. (g) HCT116 cells, which harbor a KRAS-G13D mutation, were infected with shRNA targeting GFP or FAM83B and injected subcutaneously into immunocompromised mice to assess tumor formation.

Article Snippet: Phase contrast images were taken at day 12 and total acini were quantified using an automated multi-panel scanning microscope, and the digital images were analyzed using MetaMorph image quantification software.

Techniques: Western Blot, Expressing, Membrane, Quantitation Assay, Software, Mutagenesis, Infection, shRNA, Injection